Rearrangements occurring at the chromosomal region 11q23 (MLL breakapart) usually correspondent to a t(4;11) type translocation and involve the AF4 and MLL genes, observed in chronic lymphocytic leukemia (CLL), in acute lymphocytic leukemia (ALL), in acute myeloid leukemia (AML) and in myelodysplastic syndromes (MDS). The translocation is detected through FISH, using specific custom-labeled DNA probes, from at least 100 bone marrow cell nuclei.
This test may also be applied in combination with:
- Karyotype analysis of bone marrow cells, combined with molecular detection of multiple chromosomal rearrangements
- Multicolor karyotype (M-FISH)